Monday , March 25 2019
Pap Smear Test

How to do Pap Smear Test


  • Inspect vagina and cervix.
  • Obtain a specimen of cervical cells for cancer screening.

Definition-Pap Smear

Pap Smear is a screening test, especially for cervical cancer, in which a smear of cells exfoliated or scraped from the cervix or vagina is treated with Papanicolaou stain and examined under a microscope for pathological changes. Also called Papanicolaou smear, Papanicolaou test, Pap test.

Applicable Areas

Gyneac & Obstetric Department and Gyneac & Obstetric Out Patient Department.


Consultant, Medical Officer, Staff Nurse, Auxillary Nurse Midwife and Head Nurse.


  • Light Source.
  • Vaginal speculum.
  • Gloves
  • Cotton balls.
  • Slides
  • Wooden or plastic spatula


  • Wash hands with following hand hygiene steps.
  • Prepare equipment beside exam table.
  • Explain procedure to woman for hr cooperation.
  • Instruct mother to empty her bladder before starting the procedure.
  • Assist woman into lithotomy position.
  • Cover woman with half bedsheet to maintain privacy.
  • With gloved hands inspect, palpate the external genitalia/perineal area.
  • Insert sterile speculum into the vagina.
  • Open the introitus with the help of speculum.
  • Oblique insertion of speculum into the vagina.
  • After final insertion of speculum inside the vagina.
  • Opening the blades of speculum and screwed it.
  • Excess mucus is removed from the cervix with dry cotton or wooden/plastic spatula.
  • A saline moistened Dacron applicator is introduced into the endo-cervical canal and rotated 360O and rolled on slide.
  • A wooden or plastic spatula is used to obtain the recto cervical sample.
  • The longer end is introduced into the cervical as pressed and turned in a full circle.
  • The specimen is smeared on a glass slide.
  • Labelled the slide with woman name.
  • Give comfortable position to the woman and help her to get down from examination table.
  • Send smear slide to the pathology department with receiving acknowledgement.
  • Clean the equipment and follow aseptic technique.
  • Wash hands with following hand hygiene steps.


Pathology Specimen Book